Methods applied

• Surgical examination of rodents, cannulation, cerebrospinal fluid and blood collection
• Stereotoxic operations, brain lesions, pathway transection
• Chemogenetics, viral injections
• Behavioral tests of emotional changes and maternal motivation of animals
• Automatic video analysis
• Continuous monitoring of behavior with Intellicage
• Neuronal pathway tracing using transgenic mice and viral techniques
• Laser microdissection, RT-PCR, real-time fluorescent PCR
• Bioinformatics of systems biological data
• RNA amplification for DNA microarray and sequencing
• In vitro electrophysiology, patch-clamp technique
• Molecular Biology Techniques
• In situ hybridization histochemistry, autoradiography
• Immunostaining and fluorescence microscopy
• Western blot techniques