Methods applied

  • Acute and chronic in vivo micro- and macroelectrophysiological methods (recording of unit-, multiunit activity, EEG, evoked potentials, etc.)

  • Juxtacellular filling of individual neurons with biocytin and immunohistochemical identification of the recorded neuron

  • Behavioral studies
  • Measurement of activities related to the circadian rhythms (drinking pattern)
  • Pharmacological manipulation of the ascending activating systems
  • Intraventricular and intraparenchymal injection methods
  • Standard histological and immunohistochemical technics

  • Wide variety of computer-based data analysis methods