Methods applied

• Acute and chronic in vivo micro- and macroelectrophysiological methods (recording of unit-, multiunit activity, EEG, evoked potentials, etc.)

• Juxtacellular filling of individual neurons with biocytin and immunohistochemical identification of the recorded neuron

• Behavioral studies
• Measurement of activities related to the circadian rhythms (drinking pattern)
• Pharmacological manipulation of the ascending activating systems
• Intraventricular and intraparenchymal injection methods
• Standard histological and immunohistochemical technics

• Wide variety of computer-based data analysis methods